Graphene related materials are attracting scientists from both academia and industry for their various advantages with interest for several applications. Among graphene forms graphene oxide (GO) and graphene quantum dots (GQDs) display advantageous characteristics with interest for building innovative biosensing platforms. This is due to their excellent capabilities ranging from easy linking to (bio)chemical/synthetic receptors to unprecedented electronic and optical properties. Quenching of the fluorescence induced by GO or photoluminescence of GQDs can easily operate in synergy with various other nanomaterials and platforms opening the way to several unprecedented biosensing strategies. In our recent publications we have explained the rationale behind the use of GO and GQDs in several optical and electrochemical biosensing technologies. Taking advantage of graphene materials we have developed simple, sensitive, selective and rapid biosensing platforms for various diagnostics applications. Coupling of graphene with simple green materials such as nitrocellulose is further pushing paper-based sensors toward cost efficient sensing technologies opening the way to future industrialization of such point of care devices with interest for human health protection, safety and security beside other uses.